Library preparation
Library preparation in genomics is the generation of DNA fragments for sequencing.
Library preparation in genomics is the generation of DNA fragments for sequencing.
Library preparation for NGS involves creating a collection of DNA fragments for sequencing. Genomic libraries for next-generation sequencing (NGS) can be prepared through fragmentation of a genomic DNA sample and ligating adapters to both ends of the DNA fragment.
The purpose of a DNA library is to create a collection of DNA fragments spanning a gene of interest, a region of the genome or the entire genome for a sequencing instrument to read the genetic codes (i.e. DNA sequence). Reading the DNA sequences of the library helps us to understand the genome under study.