Single-cell genomics examines the sequences from individual cells via next generation sequencing (NGS). It can be used to study individual cells in cancer genetics, for microbiome studies or even in pre-natal genetic diagnosis (PGD).
Single cells are isolated and amplified using a whole genome amplification method to increase the DNA copies. A library is created from the amplified material and sequenced using next generation sequencing (NGS).
Single cell genomics is useful whenever sample material is scarce. It has numerous applications in both basic research and clinical settings, including cancer genetics, microbiome studies and pre-natal genetic diagnosis (PGD). It is also useful in forensic applications.