HiPrep Sephacryl S-300 HR columns are designed for routine size exclusion chromatography of proteins, such as antibodies and serum proteins.

  • Wide fractionation range – Mr 10 000–1 500 000 for globular proteins, well suited for antibody purification
  • High capacity columns – preparative separation of proteins in sample volumes up to 5 mL or 13 mL for 16/60 and 26/60 columns, respectively
  • Convenient – prepacked Sephacryl columns are easily connected to ӒKTA systems for automated chromatography at low to medium pressure

Successful use in published literature

Sephacryl S-300 HR resin has been used for decades to separate proteins by size exclusion chromatography (SEC; also called gel filtration). Here are a few of the proteins that have been purified using these Sephacryl columns:

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Product Product Name Price
17116701 HiPrep 16/60 Sephacryl S-300 HR column, 16 mm × 600 mm 16 mm 873.00 USD Add to cart


17119601 HiPrep 26/60 Sephacryl S-300 HR column, 26 mm × 600 mm 26 mm 1 172.00 USD Add to cart


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