The PrismA ELISA kit contains all reagents required to perform the assay. This includes the PrismA ligand optimized for use in ELISA and polyclonal antibodies raised specifically against the PrismA ligand. The PrismA ELISA kit was developed with a focus on usability, robustness, and environmental sustainability.

• Matched PrismA protein A ligand included in the kit removes the need to separately source standard and create a custom protocol
• High IgG tolerance simplifies sample preparation
• Parts per billion sensitivity minimizes the risk of undetected residual PrismA ligand
• Consistently low intra- and inter-plate variability ensures reproducible data
• A robust assay with excellent buffer compatibility
• Designed work with MabSelect PrismA™ chromatography resin and Fibro™ PrismA chromatography adsorbers.

The PrismA ELISA kit is capable of detecting low amounts of ligand including at the final stages of purification. Leached protein A concentration in downstream materials is usually very low, so a highly sensitive assay is required to measure leached ligand in these types of samples.

The PrismA ELISA kit has a lower limit of detection (LLD) of 10 pg/mL and a lower limit of quantification (LLQ) of 10 pg/mL.

Learn more

It is important to have an assay that can give accurate and reproducible results over multiple experiments. Assay precision is the coefficient of variation (CV) within a single assay (intra-assay) and across several experiments (inter-assay).

Intra-assay variation was assessed from the interpolated concentration of 127 in-process sample measurements.

96% of samples had in intra-assay CV < 10%, with a mean CV of 2.88%.

Inter-assay variation was assessed from 51 samples measured in triplicate assays. 78% of samples had an inter-assay CV of < 10%, with a mean CV of 5.95%.

Protein A chromatography resin with enhanced alkaline stability and binding capacity for improved process economy in antibody purification.