Fundamentals of chromatography
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Chromatography principles
Your guide to protein purification basics, tips & tricks, and more.
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Vocabulary for chromatography
Here we provide definitions for common terminology used in chromatography
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How to combine chromatography techniques
Effectively combine chromatography techniques to obtain a powerful purification protocol.
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Vocabulary for chromatography column packing
Here, we provide definitions for common terminology used in column packing.
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Protein production
Understanding the workflow from start to finish.
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Optimization of purification process with similarity score analysis
ImageQuant™ TL software quickly compares samples an performs quantitative measurements eliminating user-bias using the novel Similarity Score tool
Chromatography techniques
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Size exclusion chromatography
Fundamentals of size exclusion chromatography.
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Affinity chromatography
Fundamentals of affinity chromatography.
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Ion exchange chromatography
Fundamentals of ion exchange chromatography.
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Hydrophobic interaction chromatography
Fundamentals of hydrophobic interaction chromatography.
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Mixed mode (multimodal) chromatography
In MM chromatography, ligands immobilized to the resin interact with the target protein molecule through multiple types of interactions
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Chromatography troubleshooting
Resolve protein purification problems when and where they happen.
Tagged protein purification
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Tagged protein purification
Purify recombinant proteins that have been engineered to include a specific peptide or protein sequence.
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His-tagged protein purification
Purify histidine-tagged proteins using affinity chromatography.
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GST-tagged purification
Purify recombinant proteins that have Glutathione S-transferase (GST) tags using affinity chromatography.
Articles
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Purification of reovirus using ÄKTA start
Efficient purification of infectious virus with ÄKTA start and HiTrap Capto Core 700
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Routine protein purification with ÄKTA go
ÄKTA go: compact chromatography system for reliable antibody and tagged protein purification.
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Gain efficiency in process development
Learn how fellow scientists use ÄKTA avant to gain efficiency in protein purification process development
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Predefined purification methods
Get started with predefined methods in UNICORN control software for quick method creation.
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Protein purification FAQs
Frequently Asked Questions about protein purification.
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Antibody purification
Purify polyclonal antibodies, monoclonal antibodies (mAb), and antibody fragments.
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How buffer pH and NaCl affect size exclusion chromatography
Design of experiments is used to study the effect of buffer pH and NaCl concentration in SEC step.
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Protein sample preparation
Your guide to protein sample preparation, tips & tricks, and more.
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Automate purification to save time in the lab
Learn how to automate multistep column purifications on the ÄKTA™ pure chromatography system.
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UNICORN™ start 1.3 software FAQ
Here you will find frequently asked questions relating to the download, installation, and upgrade of the latest UNICORN™ start software. Plus, information on software compatibility and managing the database.