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Protein purification protocol flow chart

What factors determine the protein purification strategy?

Your purity requirements depend on the intended use of the purified protein (see Table below). Also the amount of protein required for different use varies greatly, from picograms to kilograms or even tons.

Typical applications Purity level
Mass spectrometry
Antigen for immunization
Moderate to high,
80% to 90%
Functional studies
Structural studies
Very high, 95% to 99%
Structural studies
Therapeutic proteins
Highest > 99%