FAQ
See the table below for differences among the products.
Products | Glutathione Sepharose High Performance | Glutathione Sepharose 4 Fast Flow | Glutathione Sepharose 4B |
---|---|---|---|
Product codes | 17-5279-01, 17-5279-02 | 17-5132-01, 17-5132-02, 17-5132-03 | 17-0756-01, 17-0756-05, 17-0756-04 |
Base matrix | 6% highly cross-linked agarose | 4% highly cross-linked agarose | 4% cross-linked agarose |
Average bead size | 34 µm | 90 µm | 90 µm |
Binding capacity (mg rGST /ml) | 7 | 10 | 25 |
pH stability | 3-12 | 3-12 | 4-13 |
Application | Gives sharp peaks with concentrated proteins | Excellent choice for purification scale-up | High yields of the GST-tagged protein |
No, the GST-tag will denature in 6-8 M urea or 6 M guanidine HCl and will not be able to bind to the glutathione ligand. It is however possible to use 1 M urea at binding, although the yield of tagged protein will be lower.