FAQ
No. Plant cells can be difficult to lyse and also contain components that may interfere with DNA amplification.
FFPE samples typically contain fragmented DNA that is difficult to amplify representatively. Phi29 DNA polymerase works optimally on intact, high molecular weight template and is not suitable for use with FFPE samples.
Yes. We provide protocols for use with both gram negative and gram positive bacteria. The high sensitivity of Single Cell GenomiPhi makes it ideal for use with a single bacterial genome.
The maximum sample volume is 2 µl. The lysis reagents are carefully formulated to provide the right conditions for optimal amplification. Sample volumes above 2 µl can negatively impact both lysis and amplification performance.
Single Cell GenomiPhi has been developed to eliminate DNA contamination and improve reaction kinetics. Also, the use of lysis reagents provides optimal release of genomic DNA for improved amplification.