His-tagged Protein Purification Methods

Your guide to protein purification basics, tips & tricks, and more.

Here we provide definitions for common terminology used in chromatography

Effectively combine chromatography techniques to obtain a powerful purification protocol.

Here, we provide definitions for common terminology used in column packing.

Understanding the workflow from start to finish.

ImageQuant™ TL software quickly compares samples an performs quantitative measurements eliminating user-bias using the novel Similarity Score tool

Fundamentals of size exclusion chromatography.

Fundamentals of affinity chromatography.

Fundamentals of ion exchange chromatography.

Fundamentals of hydrophobic interaction chromatography.

In MM chromatography, ligands immobilized to the resin interact with the target protein molecule through multiple types of interactions

Resolve protein purification problems when and where they happen.

Purify recombinant proteins that have been engineered to include a specific peptide or protein sequence.

Purify histidine-tagged proteins using affinity chromatography.

Purify recombinant proteins that have Glutathione S-transferase (GST) tags using affinity chromatography.

We compared detergent-based lysis to ultrasonication and detected the emitted light using Amersham™ ImageQuant™ 800 imaging system.

Efficient purification of infectious virus with ÄKTA start and HiTrap Capto Core 700

ÄKTA go: compact chromatography system for reliable antibody and tagged protein purification.

Learn how fellow scientists use ÄKTA avant to gain efficiency in protein purification process development

Get started with predefined methods in UNICORN control software for quick method creation.

Frequently Asked Questions about protein purification.

Purify polyclonal antibodies, monoclonal antibodies (mAb), and antibody fragments.

Design of experiments is used to study the effect of buffer pH and NaCl concentration in SEC step.

Your guide to protein sample preparation, tips & tricks, and more.

Learn how to automate multistep column purifications on the ÄKTA™ pure chromatography system.

Here you will find frequently asked questions relating to the download, installation, and upgrade of the latest UNICORN™ start software. Plus, information on software compatibility and managing the database.