HiTrap™ protein L antibody fragment purification columns
HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain.
Pack size
Overview
Want to learn about our latest protein L resin? Check out HiTrap™ column with MabSelect™ VL resin, with higher binding capacity and more robust alkaline stability than Capto™ L resins.
HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain.
- Suitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (dAbs), and single-chain variable fragments (scFvs).
- Enables a platform approach to antibody fragment purification.
- High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
- Convenient for process development, as well as small-scale purification.
- HiTrap™ format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system.
Protein L chromatography column
HiTrap™ Protein L column is packed with Capto™ L resin. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody, scFv, and Fab purification.
Successful use in published research
HiTrap™ Protein L is used in a variety of research applications, including:
- Characterization of a human colorectal cancer MDR1/P-gp Fab
- Purification optimization of an scFv against type 1 insulin-like growth factor receptor (IGF-1R)
Other Cytiva Capto™ L chromatography columns and formats
Capto™ L resin is also available in the following formats:
- Bulk Capto™ L resin
- HiScreen Capto™ L prepacked column, for method and process development
- PreDictor Capto™ L 96-well plate for high-throughput screening or process development.
