Biodyne and Immunodyne nylon transfer membranes
Nylon membranes with high sensitivity and low background for radioactive and non-radioactive detection methods.
Biodyne and Immunodyne nylon transfer membranes
Nylon membranes with high sensitivity and low background for radioactive and non-radioactive detection methods.
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Overview
Biodyne and Immunodyne nylon membranes provide high sensitivity, low background, and lot-to-lot consistency for radioactive and non-radioactive detection methods. Their hydrophilic nature results in easy wetting across the membrane. When subjected to multiple cycles of hybridization, stripping and reprobing, the stability and durability of our nylon membranes is showcased as they will not crack, shrink, or tear. Available in five chemistries providing versatile adsorption properties including: Amphoteric, Positively-Charged, Positively-Charged with High lsoelectric Point, Negatively-Charged and Modified.
- BioDyne A: Amphoteric Nylon 6,6 - The net charge of this membrane can be modulated by changes in pH. It is ideal for single probe or multiple rehybridizations and in applications where background is troublesome. The binding mechanism for this membrane is through hydrophobic and electrostatic interactions. Nucleic acids can be immobilized to this membrane via UV crosslinking and baking. It is well suited for nucleic acid dot blots, gene probe assays, and DNA fingerprinting.
- Biodyne B: Positively-Charged Nylon 6,6 - The pore surfaces of this membrane are populated by a high density of quaternary ammonium groups. It offers high sensitivity in nucleic acid detection applications. The binding mechanism for this membrane is through electrostatic interactions. Nucleic acids can be immobilized to this membrane via UV crosslinking and baking although it is not required. It is well suited for nucleic acid dot blots, DNA fingerprinting, and colony/plaque lifts.
- Biodyne Plus: Positively-Charged Nylon 6,6 with High lsoelectric Point - This membrane has an extremely high isoelectric point which provides greater sensitivity than the amphoteric nylon while exhibiting lower background than the standard positively-charged nylon membrane in certain non-radioactive detection systems. It is our most sensitive nylon membrane for nucleic acid detection. The binding mechanism for this membrane is through electrostatic interactions. Nucleic acids can be immobilized to this membrane via UV crosslinking and baking although it is not required. It is well suited for nucleic acid dot blots and DNA fingerprinting.
- Biodyne C: Negatively-Charged Nylon 6,6 - The surface of this membrane is covered with a high density of carboxyl groups that can be derivatized for protein attachment. The binding mechanism for this membrane is through electrostatic interactions. It is well suited for protein immobilization, ELISAs, and affinity purification via ligand attachment.
- Immunodyne ABC: Modified Nylon 6,6 - This membrane is surface modified through proprietary chemistry which allows for covalent binding and stable ligand immobilization. The membrane is preactivated to form covalent linkages with nucleophilic groups found on proteins and other biological macromolecules. Primary reactivity is with amine groups at neutral pH. The microporous structure of the membrane provides an available immobilization area of up to 300 cm2 for each cm2 of planar membrane. It is intrinsically hydrophilic and exhibits instantaneous wetting on contact with low ionic strength aqueous solutions. High capillarity and rapid absorbent wicking are obtainable without the necessity of surface modifying wetting agents. Prewetting is not required prior to contact with ligand solutions. This membrane offers greater binding capacity for proteins than traditional non-porous solid phase surfaces.
Product specifications
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Showing 31 products
Biodyne A membrane
60106
In stock51 available
Biodyne A membrane
60113
In stock13 available
Biodyne A membrane
BNNF810S
In stock26 available
Biodyne A membrane
BNRG810S
In stock8 available
Biodyne A membrane
BNXG810S
In stock8 available
Biodyne B membrane
BNBZH50001
Stock on request
Biodyne A membrane
BNRGR15240
Stock on request
Biodyne A membrane
BNRGR500
Stock on request
Biodyne A membrane
BNXF810S
Stock on request
Biodyne A membrane
BSP0533
Stock on request
Biodyne B membrane
60200
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Biodyne B membrane
60201
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Biodyne B membrane
60207
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Biodyne B membrane
60208
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Biodyne C membrane
60314
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Biodyne C membrane
60315
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Biodyne C membrane
60336
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Biodyne Plus membrane
60400
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Biodyne Plus membrane
60404
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Biodyne Plus membrane
60406
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Immunodyne ABC membrane
BC045H810S
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Immunodyne ABC membrane
BC120H810S
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Biodyne C membrane
BNBCH810S
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Biodyne B membrane
BNBZF810S
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Biodyne B membrane
BNBZH3R
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Biodyne B membrane
BNBZH50006
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Biodyne B membrane
BNHZF810S
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Biodyne C membrane
BNNCH810S
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Biodyne C membrane
BSP0193
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Biodyne B membrane
BSP0616
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Biodyne B membrane
BSP0687
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