Butyl Sepharose™ High Performance
Butyl Sepharose High Performance is an aliphatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority
Pack size
Overview
- Aliphatic HIC resin designed for intermediate purification and polishing steps
- Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size
- Relatively high ligand density for high dynamic binding capacity even at relatively low salt concentrations
- BioProcess resin supported for industrial applications
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
Butyl Sepharose High Performance is a member of the Cytiva range of HIC resins for intermediate purification and polishing of proteins. Butyl Sepharose High Performance is based on highly crosslinked, 34-µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. It is a truly hydrophobic resin displaying a minimum of ionic interactions. Butyl Sepharose High Performance resins is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
Butyl Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Butyl Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support
