Request the sequence of Cytiva™ Protein Select™ tag now

A simple purification protocol

Purifying a protein tagged with Cytiva™ Protein Select™ tag is simple. One chromatography step does the affinity purification, a traceless tag cleavage, and the tag removal. The pure protein obtained is in its native state with no traces of the tag amino acids. The protocol does not require the use of a protease, and the same buffer can be used for equilibration, binding, wash, and elution.

Formats for research through to commercial manufacturing

Cytiva™ Protein Select™ resin is available in 25 mL, 100 mL, 500 mL, 1 L, and 5 L packs of bulk resins and in HiTrap™ 1 mL and 5 mL columns, which are well suited for research, process development and scaling up to commercial scales. Packing methods for Tricorn™, HiScale™, or AxiChrom™ chromatography columns are available.

The resin is shipped in 20% ethanol. We offer other custom prepacked column formats on request, including custom packed ReadyToProcess™ columns. Contact your Cytiva sales representative for more information.

Regulatory support files (RSF) will be available shortly at cytiva.com/RSF. For a Cytiva™ Protein Select™ ligand restricted license, contact your Cytiva sales representative for assistance and updates on availability.