Cytiva™ superSEC resin
Cytiva™ superSEC resin is a size exclusion chromatography (SEC) resin designed for purification of large biomolecules such as exosomes.
Pack size
Overview
Cytiva™ superSEC resin is part of our Custom Designed Media program. It is an alternative to Sepharose™ CL-2B, which purifies exosomes at small scale. Cytiva™ superSEC resin is suitable for much higher flows, up to 300 cm/h at 20 cm bed height (<200 kPa).
- Efficient industrial-scale purification of large biomolecules based on our well-established platform of size exclusion chromatography (SEC) resins
- Flexible process design due to large operational window of flow rates and bed heights
- Shorter chromatography cycle time and, consequently, improved productivity compared with Sepharose CL-2B
- Excellent chemical stability
Cytiva™ superSEC resin is based on an agarose base matrix with good pressure-flow properties and relatively large porosity. The pore size distribution makes the resin suitable for fractionation of exosomes and similarly sized entities from smaller sized impurities, like host cell proteins and enzymatically degraded DNA. The features of the base matrix make Cytiva™ superSEC resin an excellent choice for high-productivity purification of large biomolecules. The hydrophilic nature of the resin minimizes nonspecific adsorption and maximizes recovery of the desired biomolecule or entity.
Guidance about column packing is found in the application note. Prepacked columns for R&D use or use under GMP can be found here. See custom column packing or reach out to your local Cytiva representative for more information.
