The dissociation enzyme mix D (kidney) is intended for dissociation of kidney tissue for single-cell workflows. It has not been tested with other tissue types.
Dissociation enzyme mixes
Four tissue dissociation enzyme mixes for lung, liver, brain, and kidney tissues optimized for manual and semi-automated single-cell workflows.
Dissociation enzyme mixes
Four tissue dissociation enzyme mixes for lung, liver, brain, and kidney tissues optimized for manual and semi-automated single-cell workflows.
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Overview
Today, tens of thousands of individual cells from asingle tissue sample or patient can be analyzed,giving researchers the opportunity to understandindividual cell populations and their behavior indiseased tissue. One of the first, and most critical,steps in single cell analysis is the dissociation oftissues to yield fully disaggregated but intact andviable cells, which is often performed with tissuedissociation enzymes. Effective tissue dissociationprotocols are necessary to complete single-cellanalysis so that targeted treatments can bedeveloped for lungs, liver, brain, and kidneys.
Our QC validated tissue dissociation enzyme mixes*are ready-made solutions for dissociation of lung,liver, brain, and kidney tissues. The enzyme mix kitscan be used in manual or semi-automated tissuedissociation protocols and are effective at 4⁰C or37⁰C**. Key features include:
- Improved single cell viability and reduced cellular aggregation.
- Low variability with minimal hands-on time.
- Dissociates segments that are operative in each tissue.
- Optimized for manual tissue disaggregation techniques and semi-automated** techniques using the VIA Extractor™ tissue disaggregator.
*For research use only.
**Performance data is not yet available for Enzyme mix D (for kidney) using manual methods or at temperatures of 37⁰C. Enzyme mix B (for liver) is recommended for use at 37⁰C as liver is very sensitive to cold. Performance data is not available for Enzyme mix A (for lung) using semi-automated methods at temperatures of 37⁰C. Performance data is not available for Enzyme mix C (for brain) using semi-automated methods at temperatures of 37⁰C. Data is available for all other enzymes in the range.
***For manual dissociation protocols, enzyme mixes A, B, and C are sufficient for 25 reactions each, while enzyme mix D is sufficient for 20 reactions.
Product specifications
Showing 4 products
Dissociation enzyme mix D (Kidney)
29733434
Selling fast3 available
Dissociation enzyme mix C (Brain)
29740240
Selling fast2 available
Dissociation enzyme mix A (Lung)
29740243
Selling fast5 available
Dissociation enzyme mix B (Liver)
29751004
Selling fast5 available
Product availability is not guaranteed and subject to change.
