HiLoad™ Superdex 75 pg preparative size exclusion chromatography columns

HiLoad Superdex 75 pg prepacked columns are for high-resolution size exclusion chromatography of recombinant proteins from samples up to 5 mL or 13 mL.

Column i.d.

16 mm

26 mm

HiLoad Superdex 75 pg columns

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Overview

HiLoad Superdex 75 pg prepacked columns are for high-resolution size exclusion chromatography of recombinant proteins from samples up to 5 mL or 13 mL.

Prepacked format – for convenience and reproducibility; can be used with standalone pump or chromatography system.
High resolution – for high purity.
Scalable – separations can be scaled up using Superdex 75 prep grade resin.
ÄKTA system compatible – Fittings (1/16”) for direct connection to ÄKTA systems and other chromatography systems.

Gel filtration column selectivity

This column is packed with Superdex 75 prep grade (pg) resin. This SEC resin combines excellent mechanical strength with high hydrophilicity, allowing high flow rates and minimal nonspecific interactions. Steep selectivity curves give excellent resolving power for proteins and other biomolecules in the molecular weight range: M_r ~ 3000 to 70 000.

Successful use of Superdex 75 columns in research

These size exclusion columns are well suited to many applications, including:

Purification of helicase RHAU proteins to characterize G-quaduplex recognition
Purification of bacteriophage proteinsfor crystallization to study Cas3 inhibition
Isolation of botulinum toxin proteins to study breast cancer cell activation

Other Cytiva size exclusion chromatography columns

Cytiva offers a variety of Life Sciences solutions for gel filtration (size exclusion chromatography). Among these are columns for analysis, small-scale purification, or separation across a broader molecular weight range.