MabSelect™ VL protein L resin in HiScreen™ chromatography columns. A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability.
As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L resin can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1.
MabSelect™ VL protein L resin showed a two-fold increase in binding capacity compared to our Capto™ L chromatography resin. This higher capacity provides productivity advantages in research and biomanufacturing.
The MabSelect™ VL protein L resin can withstand cleaning with 0.1 M NaOH, thanks to multimerization and modification of one of the domains of the native protein L ligand.
HiScreen™ columns are convenient for method optimization and parameter screening enabled by the 10 cm bed height for efficient chromatography process development. MabSelect™ VL protein L resin is also available in 1 mL and 5 mL HiTrap™ prepacked columns, bulk resins in different pack sizes, PreDictor™ RoboColumn® minicolumns, and ReadyToProcess™ columns for purifying protein as well as screening and process evaluation. Contact your Cytiva sales representative for more information.
