MabSelect™ protein A affinity resin
MabSelect™ resin is a BioProcess™ protein A resin for capturing monoclonal antibodies (mAbs) from large sample volumes.
Pack size
Overview
MabSelect™ resin is a BioProcess™ protein A resin for capturing monoclonal antibodies (mAbs) from large sample volumes.
- High-flow agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
- Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity, which reduces resin volume requirements.
- Low non-specific binding resulting in low levels of impurities in the product eluate pool
- BioProcess™ resin supported for industrial applications and well-established in approved processes.
- Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
Optimized for high capacity and throughput
The resin is based on a highly cross-linked agarose matrix with a recombinant protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity resin with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. MabSelect™ resin is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.
High purification factor in a single step
The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similarto that of native protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect™ resin an excellent choice for purification of mAbs at process scale.
Security and support
As a member of the BioProcess™ resins range, MabSelect™ resin meets industrial demands with security of supply and comprehensive technical and regulatory support.
Prepacked columns and other formats
MabSelect™ resin is available in the following formats:
Process development scale – prepacked
- MabSelect™ resin in PreDictor™ plates – 96-well plates for high-throughput screening or process development.
- MabSelect™ resin in PreDictor™ mini columns – minitiaturized column format for high throughput process development.
- MabSelect™ resin in HiScreen™ columns – optimized for method and process development.
Lab/research scale – prepacked for manual purification
MabSelect™ resin in HiTrap™ columns – for easy use with a syringe, peristaltic pump, or chromatography system.
Lab/research scale – prepacked for automated purification and analysis
MabSelect™ resin in HiTrap™ columns – for easy use with a syringe, peristaltic pump, or chromatography system.
Process scale purification – prepacked
MabSelect™ resin in ReadyToProcess™ NS columns – 1 L and larger columns for clinical manufacturing that avoid the need for cleaning and cleaning validation.
Learn about prepacked MabSelect™ resin in HiScreen™ columns, MabSelect™ resin in HiTrap™ columns, MabSelect™ resin in ReadyToProcess™ columns, MabSelect™ resin in PreDictor™ plates or MabSelect™ resin in PreDictor™ mini columns
