Small vial of NHS-Activated Sepharose 4 Fast Flow resin from Cytiva, used for protein coupling and affinity purification.

NHS-Activated Sepharose 4 Fast Flow resin bottles from Cytiva used for high-capacity protein immobilization.

NHS-Activated Sepharose 4 Fast Flow resin

High-performance NHS-activated resin for efficient covalent coupling of proteins, peptides, or other ligands.

Pack size

25 mL

500 mL

To buy, choose your product.

Price includes standard shipping and handling

Overview

NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.

NHS pre-activated resin for coupling of small amino-containing proteins and peptides in process-scale applications
High level of pre-activation gives a high degree of substitution of the selected ligand
NHS coupling method results in chemically stable ligand attachment
BioProcess resin supported for industrial applications

NHS-activated Sepharose 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.

This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS-activated Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, NHS-activated Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

Product specifications

Showing 2 products

17090601

NHS-Activated Sepharose™ 4 Fast Flow affinity resin, 25 mL

25 mL

In stock13 available

344.00 USD

17090602

NHS-Activated Sepharose 4 Fast Flow, 500 mL

500 mL

Selling fast2 available

5,247.00 USD

Product availability is not guaranteed and subject to change.

Product support

FAQ

What is NHS-Activated Sepharose 4 Fast Flow used for?

NHS-Activated Sepharose 4 Fast Flow is used to couple ligands with primary amino groups to create affinity chromatography resins. It is particularly suited for immobilizing small protein and peptide ligands in process-scale applications.

How do I couple proteins to NHS-activated resins?

To couple proteins, wash the resin to remove isopropanol, then mix it with the ligand containing primary amino groups. The NHS group on the resin forms a stable amide bond with the ligand.