Ready-To-Go T4 DNA Ligase
Catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl and the 3′-hydroxyl group of two double-stranded DNA fragments.
Ready-To-Go T4 DNA Ligase, 50 reaction beads
27036101
Stock on request
Price includes standard shipping and handling
Overview
Ready-To-Go T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments.
- Covalently-linking DNA fragments takes only 30 minutes.
- Single-dose, ambient temperature stable reaction mixes.
Ready-To-Go T4 DNA Ligase includes self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA.
Adenosine triphosphate (ATP) is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.
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27036101
50 reactions
Stock on request
Product availability is not guaranteed and subject to change.