1. Particle size grade
2. Volume
Sephadex™ G-25 is well established gel filtration resin for desalting and buffer exchange in industrial applications
Sephadex resins are prepared by cross-linking dextran with epichlorohydrin. The resins are supplied dry, except when prepacked in columns and plate format. The resins swell in aqueous solutions (see data file). Sephadex G-25 is one of six different Sephadex G-types. The different types of Sephadex resins differ in their degree of cross-linking and hence in their degree of swelling and molecular fractionation range.
The four grades (Coarse, Medium, Fine and Superfine) form the basis of a range of group separation products. Coarse and Medium grades are preferred for group separations at process scale where high flow rates and low operating pressures are required. The Fine and Superfine grades are for routine lab work including preparative separations. The smaller particle sizes of the Fine and Superfine grades give shorter diffusion distances and allow for highly efficient separations at high flow rates.
| Sephadex™ G-25 | Dry particle diameter (μm) | Diameter ration (hydrated:dry) |
| Superfine | 20 to 50 | ~ 1.7 |
| Fine | 20 to 80 | ~ 1.7 |
| Medium | 50 to 150 | ~ 1.7 |
| Coarse | 100 to 300 | ~ 1.7 |
Sephadex G-25 is available in different bulk pack sizes and convenient pre-packed plate and column formats. Popular products include:
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