Capto HiRes Q and Capto HiRes S are strong anion and strong cation exchangers, respectively with ion exchange properties that remain constant during chromatography within the recommended pH range of 2 to 12.

A high-performance IEX column format

Capto HiRes Q and Capto HiRes S IEX resins are prepacked in high-performance Tricorn glass columns of two different sizes. The columns have fittings for simple connection to ÄKTA chromatography systems or equivalent high-performance systems. All column parts are biocompatible.

High-resolution protein purification

In many research areas, for example in structural biology using X-ray crystallography or cryo-electron microscopy (cryo-EM), obtaining homogeneous size and charge of biomolecules is crucial for the elucidation of their structures. High-resolution separation of samples based on their charge properties is essential to secure sample charge homogeneity and success of the study. Capto HiRes columns separate proteins and biomolecules that are very close in net charge, which makes these columns great for these applications.

Protein analysis

Capto HiRes ion exchange columns can be used for protein analysis, in applications such as characterization of target protein charge variants.

Capto Q HiRes and Capto S HiRes replace MonoBeads columns

A separation that worked on a Mono Q or Mono S column may be performed on a Capto HiRes Q or Capto HiRes S column with little modification or optimization. Similar resin selectivity and slightly improved resolution can be expected with the Capto HiRes columns while using the same experimental conditions. The similar selectivity of the two columns ensures a smooth transition even for quality control (QC) applications.