Activated Thiol Sepharose 4B is a resin used for reversible immobilization of molecules containing thiol groups under mild conditions. Optimized for immobilization of large molecules

  • Reversible coupling of proteins and large biomolecules with thiol groups to Sepharose 4B via a glutathione spacer arm
  • The ligand is a mixed disulphide formed between 2,2'-dipyridyl disulphide and glutathione coupled to CNBractivated Sepharose 4B.
  • Well suited for covalent chromatography of large molecules such as enzymes and nucleic acids
  • Gel also reacts with heavy metal ions and with alkyl and aryl halides. Addition reactions occur with compounds containing C=O, C=C, and N=N bonds
  • Separate thiol-containing proteins from non-thiol-containing proteins

Activated Thiol Sepharose 4B is a mixed disulphide formed between 2,2'-dipyridyl disulphide and glutathione coupled to CNBr activated Sepharose 4B. Activated Thiol Sepharose 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules

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Product Product Name Price
17064001 Activated Thiol Sepharose 4B 15 g Discontinued

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