ImageQuant™ TL 10.2 analysis software

Advanced analysis software for gel and blot images, multiwell plates, and colony counting applications – New version 10.2 with novel functionalities

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ImageQuant™ TL analysis software: Overview

ImageQuant™ TL is designed to be fully compatible with Amersham ImageQuant™ 800 biomolecular imager and Typhoon™ imaging system.

ImageQuant™ TL supports a wide range of file formats such as TIF, JPG, IMG, and GEL and is available for both Windows® and Mac® computers.

Modules available on ImageQuant™ TL:

Gel and Blot image analysis with lane/band detection, background subtraction, quantity calibration, normalization, and lane comparison tools.
Analysis Toolbox with drawing tools for general image analysis.
Array Analysis for analysis of multiwell plates and arrays.
Colony Counter to detect and analyze images of bacterial colonies.

A trial software package is available to download. Please contact us for a 21-day trial demo license.

Download IQTL for Windows®
Download IQTL for macOS®
Download IQTL GxP for regulated labs

3D Intensity view

ImageQuant™ analysis software offers a 3D image to provide a sharp overview of the different band intensities, directly related to sample concentration. This view can be expanded in the software to full-screen for further detail.

New! Connect chromatograms to SDS-PAGE gel

ÄKTA™ Chromatogram Import function relating each fraction with its corresponding lane on the SDS-PAGE gel
DPI different Image Export options to meet scientific journals image submission requests
Deep Zoom-In tool to evaluate intensity values at pixel level and distinguish among the closest details

ImageQuant 11 Chromatogram connect fig 11

Normalization made easy

IQTL includes multiple normalization options to meet your needs for both single and multichannel images.

Single band normalization: Normalizes all bands against a single chosen reference band.
Total protein normalization: Uses the total lane volume of all bands in the lane of a selected reference channel to normalize the bands in the same lane of other channels. For multiplex images.
Housekeeping protein normalization: Identifies the largest band in the reference channel as the housekeeping band, then normalizes each lane against the housekeeping band as reference. For multiplex images.
In-channel normalization: Calculates relative volume of bands to the volume of a reference band or user-selected reference lane.

Comparison feature: Generate robust data on lane similarity

The Comparison function analyzes and determines lane similarity using mathematical modeling.
We designed the comparison feature to analyze lanes and determine their similarity to generate even more robust data.
Use the compare function to:

Compare purified samples of biopharmaceuticals to reference samples.
Determine sample purity of in batch release of drugs.
Select a specific fraction from a chromatography run by comparing lanes of different fractions to each other.
Identify any artifacts or odd spikes in lanes.
Perform gel shift assays and DNA fragment analysis.
Analyze complex samples like cell lysates.

ImageQuant™ TL GxP for regulated environments

Full data integrity and traceability with GxP module for ImageQuant™ TL
Robust password access with Windows based authentication
Audit trails with complete user activity, login attempt, and detailed analysis logs
Client-server approach to data protection for access to secure folders
Electronic signatures for approvers
Emergency login capability
Module with check feature to confirm image integrity before analysis

Array Analysis and Colony Counter modules

Analyze images of microplates, dot blots, and micro arrays using the Array Analysis module. Normalize, draw multiple grids, and more!
Analyze spots that are not in a regular array format using the new colony counter module. Designed for microbial colonies on plates, it can also be used for basic 2D analysis of gels.

ImageQuant™ TL analysis software how-to videos

Get an overview of our ImageQuant™ TL analysis software. Key features include 3-D intensity view for band analysis with normalization options, comparison features for lane similarity, array analysis, colony counter, and GxP module for regulated environments.