Our automated oligo synthesizers cover process development to full-scale manufacturing.
Oligonucleotides (or oligos) are short, synthetic strands of DNA or RNA that can be used as therapies to prevent gene expression or hinder protein function by binding to a target gene or protein sequence. The fast-growing oligo therapeutics market includes highly targeted antisense DNA and small interfering RNA (siRNA) and holds promise for treating cancers, genetic diseases, and other conditions.
Your oligonucleotide workflow starts with flow-through solid-phase synthesis, in which the oligo chain is built from nucleoside phosphoramidites in a cyclic pattern starting from solid supports. We offer systems for automated oligo synthesis at a range of scales for easy scale up and transfer, reducing time to your next milestone.
Once synthesized, the oligonucleotide sequence is cleaved from the solid support. You can choose to remove the 5ʹ-protective dimethoxytrityl (DMTr) group during this step or later during purification to enrich full-length oligonucleotide in reverse-phase chromatography (RPC) or hydrophobic-interaction chromatography (HIC).
We build dedicated hardware solutions for cleavage and deprotection. Learn more about Customized BioProcess™ Solutions.
Fully deprotected (DMT-off) oligos can be purified by high-resolution anion exchange chromatography (AIEX). DMTr-on oligos can be enriched by RPC or HIC, thereafter the DMTr group is removed at low pH and the oligo purified further by AIEX. The AIEX step ensures oligos have the desired counter-ion.
Buffer exchange removes excess salts and minimizes yield loss after IEX. Size-exclusion chromatography is a good option for desalting at research scale. For process development and manufacturing, tangential flow filtration (TFF) is typically used for buffer exchange. We offer single-use hollow fiber cartridges for efficient and aseptic TFF.
Oligo therapeutics can target previously untreatable diseases. We spoke with some scientists and patients close to these therapies.Listen to Discovery Matters podcast
Solutions for oligonucleotide synthesis at any scale