Developments in cell expansion

In the cell expansion phase in cell processing isolated T-cells that have undergone genetic modification need to be expanded to target concentrations. Discover more about T-cell expansion by downloading our posters below.

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Xuri Cell Expansion System W25

Click here for a brief overview on the benefits of rocking and the Xuri Cell Expansion System W25 technolgy.

EXPANSION OF T-CELLS USING THE XURI CELL EXPANSION SYSTEM W25 AND WAVE BIOREACTOR 2/10 SYSTEM

Data presented in this poster demonstrate the equivalent yield and functional performance of cells expanded in the WAVE 2/10 and Xuri W25 systems using comparable culture protocols. The expanded T cells remain biologically functional and can be re-activated to produce high amounts of cytokines.

Authors: Ray Ismail, Michelle Janas, Sarah Stone, Angela Marenghi, and Vincent Sauvage
Cytiva, Forest Farm, Whitchurch, Cardiff, Wales, UK


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Expansion of T-cells using the Xuri Cell Expansion System W25 and WAVE Bioreactor 2/10 System

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THE ROLE OF PERFUSION IN MAINTAINING HIGH DENSITY T-CELL CULTURES

Manufacturing for cell therapies has adopted many of the principles of the bioprocess industry, including the use of bioreactors for cell cultivation. Rocking platform bioractors are one example, where the combination of rocking agitation and perfusion media exchange allows high cell concentrations to be reached. To understand the impact of media perfusion on high-density T-cell cultures in detail, we used the Xuri Cell Exapansion W25, a rocking bioreactor, to grow primary T-cells with and without media perfusion. The impact of perfusion on cell growht and viability was analysed as was the role of perfusion in controlling the key metabolites and growth factors.

Authors: Ray Ismail, Michelle Janas, Sarah Stone, Angela Marenghi, and Vincent Sauvage
Cytiva, Forest Farm, Whitchurch, Cardiff, Wales, UK


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The role of perfusion in maintaining high density T-cell cultures

OPTIMIZATION OF THE ROCKING SPEED AND ANGLE OF A PERFUSION BIOREACTOR FOR T CELL CULTURE

For autologous T cell therapy to be effective, T cells sampled from a patient need to begrown in sufficient quantity before being injected back. The typical cell number requiredfor an 80 kg patient is 8.109 cells. Achieving such a dose with T flasks, while maintainingcell health, poses logistical challenges that can become intractable when the number of patients rises. This is why the Xuri Cell expansion system W5, a rocking platform whichenables automated high cell density culture in a close environment , is used instead of conventional bioreactors. After a 14 day culture with the Xuri W5, cell concentrations of 1.107cells/mL in a 1L volume have been consistently reached and cells exhibited a healthyphenotype. These experiments have been performed with rocking speed and angle set at 10 rpm, and 6° respectively.

In the study presented here, we investigate the influence of these parameters on the cell growth kinetics and health in order to select the settings that optimize T cell growth, thereby allowing for faster expansion.

Authors: Mark Briggs, Michelle Janas, Sarah Stone, Angela Marenghi, Simon Stubbs, Vincent Sauvage
Cytiva, Forest Farm, Whitchurch, Cardiff, Wales, UK


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Optimization of the rocking speed and angle of a perfusion bioreactor for T cell culture

T CELLS EXPANDED IN THE WAVE BIOREACTOR 2/10 SYSTEM MAINTAIN A HEALTHY PHENOTYPE

In this poster we present data from an investigation of the aging phenotype in T cells selectively expanded from peripheral mononuclear cells (PBMCs) using the WAVE Bioreactor 2/10 system.

Authors: K. Wernersson¹, M. L. Janas², S. Stone², A. Marenghi² 
¹Cytiva Bio-Sciences AB, Uppsala, Sweden,
²Cytiva UK Limited, Cardiff, UK.


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T cells expanded in the WAVE Bioreactor 2/10 system maintain a healthy phenotype

CLINICALLY RELEVANT T CELLS EXPANDED USING THE WAVE BIOREACTOR 2/10 SYSTEM

Lymphocytes, expanded for clinical use, often consist of a small selected starting population, which requires multiple rounds of replication to achieve therapeutic doses. By using perfusion culture with the WAVE Bioreactor 2/10 System, high cell density cultures which are sufficient for therapeutic doses, can be generated. The Cellbag™ bioreactors, used together with the WAVE system, are functionally closed, single-use bioreactors that are delivered pre-sterilized and suitable for cGMP production.

Authors: C. Mölleryd¹, K. Wernersson¹, U. Johansson², L. Adamson², T. Lövgren², M. Nyström², R. Kiessling², R. Tell², K. Markland²  
¹Cytiva Bio-Sciences AB, Uppsala, Sweden,
²University Hospital, Stockholm, Sweden


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Clinically relevant T cells expanded using the WAVE Bioreactor 2/10 system