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Flow-through immunoassays

Developing highly sensitive vertical flow or transverse flow

What is a flow-through immunoassay

What is a flow-through immunoassay?

Flow-through immunoassays, also known as vertical flow or transverse flow immunoassays, are basically “semi-dry” dot-blots. Due to miniaturization and a certain degree of integration, the volumes of sample and reagents are reduced and the assay can be performed in a simple disposable device. The reaction membrane is laid on top of an absorbent pad. When agents are applied onto the membrane they are drawn through by the absorbent which acts as a sink for all liquids used in processing of the test.

Membranes and absorbents for flow-through assays

Membranes and absorbents for flow-through assays

The degree of integration in flow-through assays is considerably less compared to lateral flow immunoassays. This makes development and production easier and may also avoid some of the most important patents which apply to immunodiagnostic assays. Find out how to choose the most suitable membranes and absorbents.

Navigating the journey for flow-through immunoassays

Infographic: Navigating the journey for flow-through immunoassays

We’ve taken our knowledge of flow-through immunoassay development and the elements needed for optimal performance and developed an infographic to explore the pros and cons of this technique.