Sanger sequencing

Enhancing the delivery of therapeutics through effective Sanger (or dideoxy) sequencing

Developed by Frederick Sanger, this chain termination method provides data accuracy and robustness, particularly in small-scale sequencing applications such as single-gene studies, routine sequencing for cloning and checking genotypes. Find out how Cytiva can support your workflow below or download the full workflow as an e-Poster.

Which products and solutions should you choose to support your sample preparation, amplification and clean-up needs?

Sample preparation

Amplification

Clean-up

Product	Product Name	Pack Size
25640010	TempliPhi 100 amplification kit, 100 reactions	100 reactions	View Product
25640050	TempliPhi 500 amplification kit, 500 reactions	500 reactions	View Product
25640080	TempliPhi Large Construct Kit	1000 reactions	View Product
28903529	TempliPhi sequence resolver kit,20 reactions	20 reactions	View Product
28903531	TempliPhi sequence resolver kit, 200 reactions	200 reactions	View Product
28904267	plasmidPrep Midi Flow Kit, 25	25
28904268	plasmidPrep Midi Flow Kit, 100	100
28904269	plasmidPrep Mini Spin Kit, 50	50	View Product
28904270	plasmidPrep Mini Spin Kit, 250	250	View Product
28964286	TempliPhi 2000 Reaction Kit	2000 reactions	View Product

Product	Product Name	Pack Size
25003962	Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP))	1000 units	View Product
25003963	Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP))	10000 units	View Product
25640010	TempliPhi 100 amplification kit, 100 reactions	100 reactions	View Product
25640050	TempliPhi 500 amplification kit, 500 reactions	500 reactions	View Product
25640080	TempliPhi Large Construct Kit	1000 reactions	View Product
27036101	Ready-To-Go T4 DNA Ligase, 50 reaction beads	50 reactions	View Product
27079804	Taq DNA Polymerase (cloned), 250 units	250 units	View Product
27079805	Taq DNA Polymerase (cloned)	4 x 250 units
27079806	Taq DNA Polymerase (cloned), 10 x 250 units	10 x 250 units	View Product
28903529	TempliPhi sequence resolver kit,20 reactions	20 reactions	View Product
28903531	TempliPhi sequence resolver kit, 200 reactions	200 reactions	View Product
28937348	Taq DNA Polymerase (cloned)	25000 units
28964286	TempliPhi 2000 Reaction Kit	2000 reactions	View Product
P-01096	High Purity dNTPs		View Product
P-02615	Amersham™ Solution dNTPs		View Product
P-03729	High Purity Solution ddNTPs
P-04739	High Purity rNTPs		View Product

Product	Product Name	Pack Size
28903445	GFX 96 PCR Purification Kit	96 well plates	View Product
28903466	GFX PCR DNA and Gel Band Purification Kit, 10 purifications	10 purifications
28903470	GFX PCR DNA and Gel Band Purification Kit, 100 purifications	100 purifications	View Product
28903471	GFX PCR DNA and Gel Band Purification Kit, 250 purifications	250 purifictions	View Product
29002088	ExoProStar, 20 reactions	20 reactions	View Product
29002090	ExoProStar, 100 reactions	100 reactions	View Product
29002091	ExoProStar, 500 reactions	500 reactions	View Product
29002092	ExoProStar, 2000 reactions	2000 reactions	View Product
29002101	ExoProStar, 5000 reactions	5000 reactions	View Product
29010358	ExoProStar 1-Step, 20 reactions	20 reactions	View Product
29010361	ExoProStar 1-Step, 100 reactions	100 reactions	View Product
29010363	ExoProStar 1-Step, 500 reactions	500 reactions	View Product
29010367	ExoProStar 1-Step, 5000 reactions	5000 reactions	View Product
29046321	ExoProStar S, 20 reactions	20 reactions
29046322	ExoProStar S, 100 reactions	100 reactions
29046324	ExoProStar S, 500 reactions	500 reactions	View Product
29046325	ExoProStar S, 2000 reactions	2000 reactions
29046326	ExoProStar S, 5000 reactions	5000 reactions
29176221	ExoProStar 1-Step, 2000 reactions	2000 reactions	View Product

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Sanger sequencing

An enduring presence in DNA sequencing

Customized Solutions