Sera-Mag Select

Relationship between endogenous TOP2-induced DSBs and cancer development

This study¹ shows a strong causal relationship between endogenous TOP2-induced DSBs and cancer development, confirming the lesions as major drivers of ATM-deficient lymphoid malignancies, and potentially other conditions and cancer types. Sera-Mag Select is used for the purification steps throughout the process.

¹Álvarez-Quilón, A., Terrón-Bautista, J., Delgado-Sainz, I. et al. Endogenous topoisomerase II-mediated DNA breaks drive thymic cancer predisposition linked to ATM deficiency. Nat Commun 11, 910 (2020) http://creativecommons.org/licenses/by/4.0/

Andrew Gane discussing custom plates with colleague

Sera-Mag Select reagent

Running a sequence through an instrument is expensive and time consuming. Unreliable PCR clean-up and size selection can negatively affect productivity and credibility. The high yield and tight size distribution of Sera-Mag Select provides better sequencing efficiency and more consistent performance for increased reliability and peace of mind.

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Case study: Sera-Mag Select in a targeted resequencing approach

Sera-Mag Select was tested in a targeted resequencing approach by Dr. rer. Nat. Stefanie Stepanow. Findings presented in this case study demonstrate that Sera-Mag Select yields high quality sequencing data with a high percentage of Q30 reads achieved.

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Standard NGS workflow in the targeted sequencing approach

Sera-Mag Select data file

Sera-Mag Select is based on the well-known solid phase reversible immobilization technology. The DNA size range isolated from the process can be tailored to suit the user’s end requirements, by adjusting the amount of reagent that is mixed with a fixed volume of sample.
DNA of 100 bp size or greater can be reliably recovered using
Sera-Mag Select.

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Customize the reagent to specific needs

To solve workflow challenges and improve performance and speed to market, it is sometimes necessary to modify the formulation. Sera-Mag Select is developed and produced at Cytiva’s manufacturing site of excellence in Cardiff, where it is possible to customize the formulation to specific needs.

Find out more about customization

Featured content

The importance of size selection in NGS workflows

High quality libraries are key to keeping NGS costs down and maximizing usable data. Take a closer look at how size selection can improve your NGS data quality.

Multiplexing in next-generation sequencing

Multiplexing provides great cost savings and maximizes the amount of usable data per run, but introduces complexity to the upstream workflow and risks to data quality. Learn how to simplify the process without compromising data quality or volumes.

Fundamentals of NGS sample preparation

In this blog we cover the fundamentals of preparing your samples for NGS, and considerations for each step. These steps include DNA extraction, amplification, library preparation, selection or purification, and quality control.

Next-generation sequencing: 3 main challenges (& solutions!) in NGS sample preparation

Sample prep can have a big impact on next-generation sequencing (NGS) outcomes. Here are some simple things you can do to get your NGS off to a good start.

Request a free sample of Sera-Mag Select PCR clean-up and size selection reagent

1 mL sample bottle which provides approximately 7 to 8 reactions based on a typical size selection application (typical sample size of 50 µL).

Selective binding and PCR clean-up with Sera-Mag Select