ÄKTA start column packages
Supporting purification of antibodies, tagged or untagged proteins.
Simplify your work with prepacked column packages
ÄKTA start is well-suited for use with HiTrap columns. To simplify your work, we offer five application-specific column packages. The packages consist of columns prepacked with high-performance media, supporting purification of tagged proteins, antibodies, or untagged (native) proteins.
Column packages for tagged protein purification
For histidine-tagged proteins, the His-tagged column package includes HisTrap HP for high yield or HiTrap TALON® crude for high purity.
The GST-tagged column package for GST-tagged proteins includes a GSTrap 4B column for high yields. HiTrap Desalting columns are provided for rapid buffer exchange.
Column packages for antibody purification
For antibody purification, choose between the Protein G or Protein A column package including HiTrap Protein A HP column for human antibodies or HiTrap Protein G HP column for most antibodies, including rat. A HiTrap Desalting column is also provided for quick pH neutralization of the eluted antibodies.
Column packages for purifying untagged or native proteins
The Untagged column package for native proteins includes HiTrap SP HP cation exchange and HiTrap Q HP anion exchange columns, to be used individually or in combination. The package also contains a HiPrep Sephacryl size exclusion column with a fractionation range suitable for most proteins.
