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There are different digital imaging technologies available for Western blot protein detection. How do you choose between using a CCD camera and a laser scanner?Continue reading
Global life sciences leader Cytiva has acquired the German scientific software maker GoSilico, in a move that will differentiate Cytiva as a premier partner in bioprocessing.
The polyhistidine-tag is the most widely used affinity tag in research involving recombinant proteins. It is small, and hence, the first choice in many protein purification workflows. Purification of his-tagged proteins is often straightforward using an immobilized metal ion affinity chromatography (IMAC) resin. Still, low or no recovery of your target protein can occur.
Based on responses from happy scientists, we are sharing these automated methods for removing an affinity tag and the protease. The tag is cleaved while the protein is still bound to the affinity resin. The next step depends on the protease. Thrombin or Factor Xa is removed by connecting a second (Benzamidine) column. Tagged proteases are removed on the same column used for capture.