7 steps to western blotting
Overview of protein immunoblotting in Western blotting for protein research.
Western blotting in 6 steps
Western blotting is a common application developed in 1979 yet still today, it is routinely used in protein research laboratories today to detect and quantify proteins. From early methods of radioactive detection to more sophisticated fluorescence based imaging, resulting in better quality data and quantification. Western blotting comprises six key steps.
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When submitting articles to a scientific journal, images representing your raw data are often required. Safe handling and easy authentication of these images is necessary in modern research.
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eLearning
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Western blotting tips
You will learn how to analyze causes and use preventive strategies for common pitfalls, such as high background and unspecific or weak bands. as well as discuss how to find appropriate antibody dilutions without trial and error.
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Introduction to total protein normalization
Learn about the importance of normalization and TPN, advantages and limitations of three common TPN approaches and a step-by-step guide to the fluorescent prelabel approach.
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Get to know laser scanners and CCD cameras
Find out how laser scanners and CCD cameras work along with basic principles of imaging modes like chemiluminescence, fluorescence, colorimetric imaging, densitometry, and phosphorimaging and which type of imaging system suits what sample type.