FAQ

Spare parts

O-rings and nets should be checked regularly for wear. Worn O-rings may not seal properly and over-used nets can affect distribution. Inspect the O-rings and evaluate the height equivalent to a theoretical plate (HETP) and peak asymmetry factor (As) to prevent poor performance due to old nets. 

We recommend you to change the nets and O-rings when needed or at least annually. Please keep a set of each on your site, for code numbers refer to the table below.

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# Product Name Product Code Price
1 PEEK Tubing, 2 m, i.d. 1.0 mm, o.d. 1/16" 18111583 64.31 USD Add to cart
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2 Support Screen for Columns, i.d. 26 mm 18937701 60.00 USD Add to cart
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3 O-Ring 20.3x 2.62 mm 28978227 65.00 USD Add to cart
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4 Net ring 20 µm 28966380 85.00 USD Add to cart
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5 Spanner wrench 28964777 95.00 USD Add to cart
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7 Accessory kit for HiScale 26 columns 28966374 95.00 USD Add to cart
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8 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 75.02 USD Add to cart
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Figure. HiScale 26 column.

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# Product Name Product Code Price
1 Adapter complete for HiScale 26 28966384 523.00 USD Add to cart
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7 Column tube assembly 28966648 885.00 USD Add to cart
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7 Column tube assembly 28966651 1 011.00 USD Add to cart
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8 Fingertight Stop Plug, 1/16'' 11000355 59.54 USD Add to cart
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9 Union 1/16" female-1/16" female 11000339 127.92 USD Add to cart
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11 Fingertight Connector 1/16" Male 18117263 63.11 USD Add to cart
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22 O-Ring 20.3x 2.62 mm 28978227 65.00 USD Add to cart
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24 Support Screen for Columns, i.d. 26 mm 18937701 60.00 USD Add to cart
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25 Net ring 20 µm 28966380 85.00 USD Add to cart
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Accessory kit for HiScale 26 columns 28966374 95.00 USD Add to cart
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Accessories

 

 

Make sure you have all connectors and tubing needed for packing and running the column.

Depending on your system configuration and column length, you may need extra long tubing to connect your column.

2x Fingertight stop plug 1/16", 2x Union 1/16" female-1/16" female and 2x Fingertight connector 1/16" male are supplied with the column.

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# Product Name Product Code Price
1 PEEK Tubing, 2 m, i.d. 1.0 mm, o.d. 1/16" 18111583 64.31 USD Add to cart
Get Quote
2 Fingertight Connector 1/16" Male 18117263 63.11 USD Add to cart
Get Quote
3 Union 1/16" female-1/16" female 11000339 127.92 USD Add to cart
Get Quote
4 Fingertight Stop Plug, 1/16'' 11000355 59.54 USD Add to cart
Get Quote
5 Column tube assembly 28966648 885.00 USD Add to cart
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5 Column tube assembly 28966651 1 011.00 USD Add to cart
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Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 75.02 USD Add to cart
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Connector 1/16" Male/Luer Female 18111251 82.16 USD Add to cart
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Short column holder, for columns 10 to 50 mm o.d. 18111317 165.51 USD Add to cart
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Long Column Holder, columns 26 mm i.d. 18112632 244.00 USD Add to cart
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Column Holder 28956282 108.00 USD Add to cart
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Column Clamp 28956319 65.00 USD Add to cart
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Packing tube 40 for HiScale 26 columns 28964505 1 380.00 USD Add to cart
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Spanner wrench 28964777 95.00 USD Add to cart
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Accessory kit for HiScale 26 columns 28966374 95.00 USD Add to cart
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O-ring 26x4 mm 28966654 67.00 USD Add to cart
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Packing tube 20 for HiScale 26 columns 28980383 1 137.00 USD Add to cart
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Make sure you have all connectors and tubing needed for packing and running the column.

Depending on your system configuration and column length, you may need extra long tubing to connect your column.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 PEEK Tubing, 2 m, i.d. 1.0 mm, o.d. 1/16" 18111583 64.31 USD Add to cart
Get Quote
2 Fingertight Connector 1/16" Male 18117263 63.11 USD Add to cart
Get Quote
3 Union 1/16" female-1/16" female 11000339 127.92 USD Add to cart
Get Quote
4 Fingertight Stop Plug, 1/16'' 11000355 59.54 USD Add to cart
Get Quote
Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 75.02 USD Add to cart
Get Quote
Long Column Holder, columns 26 mm i.d. 18112632 244.00 USD Add to cart
Get Quote
Column Holder 28956282 108.00 USD Add to cart
Get Quote

Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Poor product recovery

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Worrying peak shape

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Drifting baseline

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Poor reproducibility

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Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.
 
 

Leading peak Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As). 

Please be aware of that for small columns, less than 10 ml bed volume, the system dead volume has an impact on the column evaluation values.

Issues related to Poor packing evaluation result

General advice to achieve good performance

Before using the system make sure that:

  • The correct system has been selected in UNICORN System Control
  • The correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected
  • All connectors are free from leakage
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

Column leakage

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Unsatisfactory elution

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Back pressure increases during operation

Unusual column appearance

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Strange peaks

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