FAQ
We recommend 10 ng of input DNA for optimal results, but quantities below 1 ng will also generate amplified DNA.
Strand displacement activity of Phi29 DNA polymerase in multiple displacement amplification generates both single- and double-stranded DNA.
Both TempliPhi and GenomiPhi use Phi29 DNA polymerase, but the enzymes are formulated differently. TempliPhi is optimized for rolling circle amplification, and GenomiPhi is optimized for multiple displacement amplification.
We recommend using the PicoGreen™ assay. Spectrophotometry is discouraged, because primers and nucleotides in the formulation interfere with UV quantitation.
We recommend ethanol precipitation. Silica columns should be avoided.