Second step in mAb chromatographic process

Capto™ adhere resin can remove key contaminants such as DNA, host cell proteins (HCP), leached protein A, dimers and larger aggregates, and viruses in a single step. Together with a protein A
capture step, (using, for example, MabSelect™ SuRe resin), Capto™ adhere resin allows the design of a two-step chromatographic process for mAbs.

Part of the Capto™ adhere family

For optimal performance of Capto™ adhere, screening and optimization of loading conditions is recommended, preferably using modern high-throughput process development tools, such as PreDictor™ 96-well filter plates.

Prepacked columns and other formats

Capto™ adhere resin is available in the following formats:

Process development scale

• PreDictor™ Capto™ adhere filter plates, 96-well plates for high-throughput screening or process development.
• PreDictor™ Capto™ adhere RoboColumn units, miniaturized columns for high-throughput process development.
• HiScreen™ Capto™ adhere columns, optimized for method and process development.
• f(x) column, prepacked and precharacterized chromatography column for fast and accurate mechanistic modeling.
• Validation columns, scale-down columns for robust and reproducible process validation and process development (Tricorn™ format)

Lab scale (for manual or automated systems)

HiTrap™ Capto™ adhere columns, for easy use with a syringe, peristaltic pump, or chromatography system.

Process-scale

ReadyToProcess™ Capto™ adhere columns, 1 L and larger columns for clinical manufacturing that minimize the need for cleaning and cleaning validation.

Process characterization kits

Process characterization kits for Capto™ adhere and other resins are designed for studying the potential impact that resin ligand density might have on the process outcome.