Capto™ Core 700 multimodal chromatography resin
Novel dual-mode combines size exclusion and binding chromatography
Capto™ Core 700 multimodal chromatography resin
Novel dual-mode combines size exclusion and binding chromatography
Pack size
Overview
- Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality. This chromatography resin has both size exclusion and binding properties.
- Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
- Significantly improved productivity and higher flow rates compared with standard size exclusion (gel filtration) methods.
- Straightforward optimization due to flowthrough chromatography and robust performance.
- Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
- Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
Multimodal removal of contaminants
Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands.
Hydrophobic, positively charged ligands
The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.
Virus and exosome purification with Capto beads
Virus and exosome purification: harness core bead technology
Scaling up is simple
HiTrap and HiScreen columns can be connected in series for increased capacity and are convenient to use when developing an efficient and robust separation method.
Prepacked columns and other formats
Capto Core 700 is available in the following formats:
Process development scale – prepacked
HiScreen Capto Core 700 columns – optimized for method and process development.
Lab/research scale – prepacked for manual purification
HiTrap Capto Core 700 columns – for easy use with a syringe, peristaltic pump, or chromatography system.
Lab/research scale – prepacked for automated purification and analysis
HiTrap Capto Core 700 columns – for easy use with a syringe, peristaltic pump, or chromatography system.
ReadyToProcess Capto Core 700 columns – 1 L and larger columns for clinical manufacturing that avoid the need for cleaning and cleaning validation.
Also, learn about the Capto Core 400 resin.
