DEAE Sepharose™ Fast Flow anion exchange chromatography resin
DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification.
DEAE Sepharose™ Fast Flow anion exchange chromatography resin
DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification.
Pack size
Overview
- Well-proven weak anion exchanger developed for industrial downstream processes.
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals.
- High chemical stability allows for well proven CIP and sanitization protocols.
- Hydrophilic base matrix ensures low levels of non-specific binding, and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Streamlined scale-up
This resin is part of the Sepharose Fast Flow ion exchange platform, which is well established in industrial downstream processing. Composed of crosslinked 6% agarose beads with diethylaminoethyl (DEAE) weak anion exchange groups, the resin has high chemical stability, allowing well-proven cleaning-in-place (CIP) and sanitization protocols.
The highly crosslinked agarose resin enables high flow rates and high productivity. Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, and PreDictor RoboColumns™, and HiTrap columns.
Successful use – research scale
DEAE Sepharose Fast Flow resin is used in a variety of research applications, including:
- Separation ofPI 3-Kinases from guinea pig neutrophils
- Purification of myosin phosphatase from chicken smooth muscle
Compatible empty columns – research scale
Cytiva's solutions include a variety of empty columns for research scale. This DEAE resin is compatible with two of these lines, Tricorn and XK columns.
Compatible empty columns – bioprocess scale
For process-scale applications for this DEAE resin, Cytiva recommends AxiChrom columns. DEAE Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Prepacked columns – research scale
This weak anion exchange resin is available in the following formats:
- HiScreen DEAE FF for scale up and process development
- HiPrep DEAE FF columns for convenient scale-up
Prepacked columns – bioprocess scale
Prepacked ReadyToProcess columns for bioprocess applications are available on request.
