Deaza dGTP
Nucleotide analog (candidate substrate) for processive telomerase, to improve PCR yields and make sequencing of GC rich sequences easier.
Deaza dGTP
Nucleotide analog (candidate substrate) for processive telomerase, to improve PCR yields and make sequencing of GC rich sequences easier.
Overview
Deaza dGTP is a nucleotide analog that is a candidate substrate for processive telomerase. Its use can improve Polymerase Chain Reaction (PCR) yields and makes sequencing of GC rich sequences easier.
- For reducing band compressions* in sequencing gels.
- Supplied as convenient solutions at pH 7.0 and 7.5. c⁷dGTP is supplied in purified water as 5 mM and 10 mM solutions.
Deaza dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase. It can also be applied to dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA. Partial substitution of Deaza dGTP for dGTP in PCR can improve the product yield when the template is GC-rich.
Technical Information:
*Band compressions are ambiguous band patterns on DNA sequencing gels. This is caused by stable, intra-strand secondary structures which are not fully denatured during electrophoresis. The likelihood of occurrence can be reduced through substitution of nucleotide analogs within sequencing mixers. To resolve G-band compressions, use 7-deaza dGTP (c⁷dGTP).
