HiTrap™ Q HP anion exchange chromatography column

Strong anion exchange chromatography column for high resolution, small-scale protein purification

HiTrap™ Q HP anion exchange chromatography column

Strong anion exchange chromatography column for high resolution, small-scale protein purification

Pack size

1 × 1 mL

5 × 1 mL

5 × 5 mL

HiTrap Q HP column

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Overview

HiTrap Q HP is a strong anion exchange chromatography column for high-resolution, small-scale protein purification.

Packed with Q Sepharose High Performance strong quaternary ammonium anion exchange resin.
Small (34 µm) bead size delivers high-performance, high-resolution purifications.
Can be used alone or connected in series.
Convenient HiTrap format designed for use with a syringe, peristaltic pump, or chromatography systems such as ÄKTA system.

Strong anion exchange resin

The Q functional group is a quaternary amino group coupled to the matrix via chemically stable ether linkages. Q Sepharose High Performance is a strong anion exchanger charged with high loading capacity over broad pH ranges.

Successful use in published research

HiTrap Q HP columns are used in a variety of applications, including:

Purification of the recombinant NbZFP1 protein to study fruit development in Nicotiana benthamiana
IEX chromatography with soluble mite extract to evaluate vaccine candidate antigens from the poultry red mite
Purification of dialysate during development of a new exonuclease from a metagenomic library

Other Q Sepharose High Performance formats

This strong anion exchanger is available in the following formats:

Bulk resin Q Sepharose High Performance
HiScreen Q HP columns for method and process development
HiPrep Q HP columns for convenient scale-up.

Other Cytiva ion exchange columns

Among Cytiva's solutions are an extensive range of ion exchange chromatography resins, lab packs, and prepacked columns, including HiTrap, HiPrep, and HiScreen formats.