Mammalian Protein Extraction Buffer is a 500 ml solution designed for efficient and gentle extraction of biologically active, total soluble proteins from mammalian cultured cells. This buffer is based on organic buffering agents, mild nondenaturing buffer, compositions and can be used for both cell suspensions as well as adherent cells. Mammalian Protein Extraction Buffer is compatible with most applications, including enzyme assays, various chromatography procedures, and electrophoresis. After preparation, the sample may be used for most enzyme assays including reporter gene assays, kinases, and immunoassays.