Rolling circle amplification and PCR kits

Amdex - Enzyme Conjugates for ELISA high performance conjugates are based on a unique chemistry - a hydrophilic straight chain dextran backbone to which many hundreds of enzyme molecules (for example, horseradish peroxidase or alkaline phosphatase) are covalently coupled.

Amdex - Enzyme Conjugates for ELISA high performance conjugates are based on a unique chemistry - a hydrophilic straight chain dextran backbone to which many hundreds of enzyme molecules (for example, horseradish peroxidase or alkaline phosphatase) are covalently coupled.

AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used.

Cy3 and Cy5 maleimide mono functional dyes are especially suitable for highly selective peptide, oligonucleotide and protein labeling via thiol maleimide coupling. 

CyDyes are a group of bright fluorescent dyes which can be used in genomic and protein research. Cy dye labelled nucleotides exhibit the following properties:

Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

Amersham CyDye Value Packs are fluorescent dyes for the labeling of free carbonyl groups of glycoproteins and carbohydrates, and are available in a range of sizes.

CyDye Value Packs (Mono-Reactive NHS Ester) contain derivatized CyDye fluors that carry only one reactive group on each dye molecule for accurate labeling of amine functional groups. Ideal for labeling antibodies, peptides, glycoproteins, and amine-rich proteins.

Megaprime DNA Labeling System allows the labeling of template DNA to the same high specific activity but at a greatly accelerated rate.

Rediprime II DNA Labeling System provides individually dispensed reaction mixes which are dried in the presence of a stabilizer and a dye to make labeling probes easier for the user. Rediprime reaction mixes have been formulated using an improved exonuclease-free Klenow to give probes with specific activity of 1.9 × 109 dpm/μg or greater after 10 minutes incubation at 37°C with the majority of DNA substrates. By replacing a nonradioactive nucleotide with the radiolabeled equivalent in the reaction mixture, newly synthesised DNA is made radioactive. Very small amounts of input DNA can be labeled, enabling very high specific activity probes to be produced with relatively small quantities of added nucleotides. These radioactive labeled fragments can then be used as sensitive hybridization probes for a wide range of filter-based applications.

Alkaline phosphatase is suitable for dephosphorylation of 5' and 3'-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, alkaline phosphatase can be easily inactivated by heating.

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Hot Start Master Mix is a 2X premixed formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR.

Preformulated and predispensed, freeze-dried PCR reagents mix including high-quality PuReTaq DNA Polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications.

Hybond N+ is a positively charged nylon membrane capable of high sensitivity in DNA and RNA blotting.

MicroSpin G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.

MicroSpin G-50 columns are designed for the rapid purification of DNA in desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions.

MicroSpin S-200 HR spin columns for rapid DNA purification of labeled single-stranded or double-stranded DNA fragments ≥50 bases in length in applications including desalting, buffer exchange, and primer removal using spin-column chromatography.