Solutions for nucleic acid isolation and RT-PCR detection
| Product | Description |
| Sera-Xtracta Virus/Pathogen Kit | Universal nucleic acid extraction kit which copurifies both DNA and RNA or just RNA and gives excellent recovery and extraction efficiency from RNA viruses such as: Influenza A (H3N2) and SARS-CoV-2. Order a sample. |
| SeraSil-Mag Silica Coated Super- paramagnetic Beads |
Silica coated superparamagnetic beads for nucleic acid isolation that deliver high purity DNA extraction for highly sensitive applications where sample is scarce. These beads provide an optimal surface for nucleic acid binding with high performance and low background. |
| RNASpin Mini Kit | RNA purification kit designed for rapid extraction of high-quality total RNA from a wide range of sample types. Includes prefilters and DNase I. |
| RNAspin Midi Kit | RNA isolation Kit designed for the rapid extraction and purification of RNA from a wide range of starting material. |
| Sera-Mag Carboxylate-Modified Magnetic Beads & SpeedBeads | Carboxylate-Modified Magnetic Beads & SpeedBeads have free carboxyl groups on the surface which form covalent amide bonds with proteins, peptides or amine-modified ligands for use in many downstream applications including protein purification, DNA/RNA sample preparation and clean-up, proteomics, and immunoassays. |
| Sera-Mag Oligo (dT)-Coated Magnetic Beads | Convenient isolation and extraction of mRNA for applications such as RT-PCR, cDNA library construction, cDNA microarrays, affinity purification, primer extension, and subtractive hybridization. |
| Poly(A) | Poly(A) is a single-stranded RNA homopolymer used to evaluate the binding of potential therapeutic agents to mRNA. |
| Nucleotides | Easy to use, high purity ddNTPs, dNTPs, and rNTPs. |
| Taq Polymerase | Highly purified thermostable recombinant DNA polymerase enzyme for PCR. |
| Cy-Dyes | Bright fluorescent dyes which can be used in genomic and protein research for labeling DNA and RNA probes. |
Please visit our online catalog for a complete list of biochemistry and molecular biology products for DNA amplification, nucleic acid and protein preparation, and DNA analysis.
Membranes for lateral flow assays
| Product | Description |
| FFHP Membranes | A high purity backed nitrocellulose membrane with reduced surfactant content. Our most hydrophobic membrane for customers looking for a highly consistent hydrophobic membrane for use with most sample types. 200 µm total thickness, including backing. |
| FFHP Plus Nitrocellulose Membranes | Our FFHP membrane but with increased surfactant content, for a less hydrophobic membrane but with the same high levels of consistency. 200 µm total thickness, including backing. |
| FFHP Plus Thick Membranes | Our FFHP plus membrane but manufactured at 235 µm thickness (including backing), creating a large pore backed membrane for easy replacement of other membranes in already developed lateral flow assays, needing minimal optimization work. |
| Immunopore Membranes | Range of plastic-backed nitrocellulose membranes with a proprietary polymer in the membrane mix, to ensure rapid rewetting and low background signal. Post treated with surfactant, so structurally different to the FFHP family of membrane. |
| AE Membranes | Range of unbacked nitrocellulose membranes for use in a lateral flow assay, for experienced users looking for a high purity but lower cost membrane. |
| Whatman Cellulose Nitrate Membrane | Cellulose nitrate membranes, circles, plain. Narrow pore size distribution for improved surface capture and analysis. Low levels of extractables to ensure sample integrity. Sold sterile and non-sterile. |
Pads for lateral flow assays
| Product | Description |
| CF1 | Light and thin 100% cotton linter material (176 µm thickness at 53 kPA), suitable for use as a sample pad for small volume lateral flow applications. |
| CF3 | Medium weight 100% cotton linter material (322 µm thickness at 53 kPA), suitable for use as a sample and absorption pad for lateral flow applications. |
| CF4 | Medium weight, 100% cotton linter material (482 µm thickness at 53 kPA), suitable for use as a sample and absorption pad for lateral flow and flow-through assays. |
| CF5 | Medium to thick weight, 100% cotton linter material (954 µm thickness at 53 kPA), suitable for use as an absorption pad for lateral flow and flow-through assays. |
| CF7 | Thick, 100% cotton linter material (1873 µm thickness at 53 kPA), suitable for use as an absorption pad for lateral flow and for use in dipstick colorimetric assays. |
| Fusion 5 | Proprietary single layer matrix membrane that can be used to replace traditional modular components from a lateral flow testing kit. |
| GF/DVA | Bound glass fiber filter, especially suitable for saliva samples. |
| MF1 | Bound glass fiber filter suitable for serum and whole blood samples (ca. 100 μl). |
| Standard 14 | Glass fiber (355 µm thickness at 53 kPA), suitable for use as a conjugate release pad in a lateral flow assay. |
| Standard 17 | Glass fiber (370 µm thickness at 53 kPA), suitable for use as a conjugate release pad in a lateral flow assay. |
Please visit our online catalog for a complete list of Whatman filters, devices, and membranes to best fit your diagnostic application.
Antigen purification
| Product | Description |
| Ni Sepharose Excel | Affinity resin for preparative purification of his-tagged recombinant antigens. Protein binding capacity is at least 10 mg (his)6-tagged protein/mL resin. |
| HisTrap Excel | Ni Sepharose excel prepacked in a convenient HiTrap column. Allows for easy connection to a chromatography system such as an ÄKTA system. |
| HisTrap HP | Ni Sepharose high performance affinity resins for preparative purification of his-tagged antigens prepacked in convenient HiTrap columns. Protein binding capacity exceeds 40 mg (his)6-tagged protein/mL resin. |
| HiLoad Superdex 75 pg Columns | Size exclusion columns used in the final step in the his-tagged antigen purification protocol to achieve highly purified antigens. Prepacked with Superdex 75 prep grade with a fractionation range of Mr 3 000 to 70 000 for globular proteins. |
| Superdex 75 Increase Columns | Prepacked columns packed with Superdex 75 Increase high resolution size exclusion chromatography resin. Convenient for quick purity check of antigens instead of laborious acrylamide gel electrophoresis with Coomassie staining. |
Antibody purification
| Product | Description |
| MabSelect PrismA | MabSelect PrismA resin for preparative purification of monoclonal and polyclonal antibodies from serum and cell culture supernatant. The Protein A ligand has been specially engineered to create an affinity resin with enhanced alkali and protease stability. The specificity of binding to the Fc region of IgG is similar to conventional Protein A and provides excellent purity in one step. |
| HiTrap MabSelect PrismA | MabSelect PrismA prepacked in convenient HiTrap columns for easy connection to a chromatography system such as an ÄKTA system. |
| Protein G Sepharose Fast Flow | Protein G Sepharose can be a complement to MabSelect PrismA for preparative purification of monoclonal and polyclonal antibodies from serum and cell culture supernatant. |
| HiTrap Protein G HP | Protein G Sepharose prepacked in convenient HiTrap columns for easy connection to a chromatography system such as an ÄKTA system. |
| HiLoad Superdex 200 pg Columns | Size exclusion columns used in the final step of an antibody purification protocol to achieve highly purified monomeric antibodies. Prepacked with Superdex 200 prep grade which has a fractionation range of Mr 10 000 to 600 000 for globular proteins; well-suited for antibody purification. |
| Superdex 200 Increase Columns | Prepacked columns packed with Superdex 200 Increase high resolution size exclusion chromatography resin. Convenient for quick purity check of antibodies instead of laborious acrylamide gel electrophoresis with Coomassie staining. |
| NHS-activated Sepharose Fast Flow | Pre-activated affinity resin for coupling amino-containing proteins and peptides. Ideal for preparation of an affinity resin with a relevant antigen as ligand. |
| HiTrap NHS-activated HP | NHS-activated Sepharose prepacked in convenient HiTrap columns for easy connection to a chromatography system such as an ÄKTA system. |
| Resource | Description |
| Article: The path for high quality immunoassay reagents. How Biacore SPR is shaping today´s assay development | Guidelines which highlight critical considerations in the development of immunoassay reagents and steps for avoiding common pitfalls, using technologies such as Biacore surface plasmon resonance. |
| Article: Epitope binning: a critical step in antibody selection | A discussion on how SPR is used to characterize closely related “bins” of mAbs targeting the same epitope of the target protein and how the selection process is used to develop new vaccines, therapeutic antibodies, and diagnostic tools. |
| Article: Converting from ELISA to Biacore SPR assays | General guidance on how to convert an existing ELISA assay to a Biacore SPR-assay and by doing so, shows how you can get results in less than half the time compared with ELISA. |
Screening and characterization
| Product | Description |
| Biacore 8K+ | Biacore 8K+ is a high-sensitivity SPR system with outstanding capacity for use in screening, characterization, process optimization, and quality control. |
| Biacore 8K | Biacore 8K is a high-throughput, high-sensitivity SPR system that enables efficient screening characterization, process optimization, and quality control of small molecules and biotherapeutics. |
| Biacore T200 | Biacore T200 is a versatile system for high-quality characterization of molecular interactions, all the way from research to discovery and quality control. |
| Biacore Insight Evaluation Software and Extensions | Software for intuitive workflow and application-specific evaluation of affinity and kinetics data generated by Biacore SPR systems. |
Antibody capture
| Product | Description |
| Human Antibody Capture Kit | Reagents for capture of human or humanized IgG antibodies as ligands in biomolecular interaction analyses. Designed for use with Biacore systems. |
| Human Antibody Capture Kit, Type 2 | Reagents for capture of human or humanized IgG antibodies as ligands in biomolecular interaction analyses. Designed for use with Biacore 8K+, and Biacore 8K. |
| Mouse Antibody Capture Kit | Reagents for the capture of mouse antibodies as ligands in various biomolecular interaction analyses. |
| Mouse Antibody Capture Kit, Type 2 | Reagents for the capture of mouse antibodies, in component volumes larger than the standard mouse antibody capture kit. Sufficient for at least 16 immobilizations and 1600 regenerations. |
| Series S Sensor Chip Protein A | Sensor chip for orientation-specific binding of antibodies to the heavy chain within the Fc region and ensures antibodies are bound to the surface in a specific orientation. Binds antibodies from several mammalian species, most notably human antibodies of the subclasses IgG1, IgG2, and IgG4. Does not bind Fab fragments (unlike native Protein A). |
| Sensor Chip Protein G | Sensor surface is preimmobilized with recombinant Protein G that binds a broad range of IgG, such as human (including IgG3), rat, rabbit, mouse, guinea pig, goat, sheep, and cow. Antibodies binds only to the heavy chain within the Fc region ensuring antibodies are bound to the surface in a specific orientation. |
| Sensor Chip Protein L | Sensor chip pre-immobilized with a recombinant Protein L for antibodies and antibody fragments containing kappa light chain subtypes (1, 3 and 4) for site-directed antibody capture without interfering with its antigen-binding site. Suitable for study of a wide range of antibody fragments including Fabs and dAbs. Carboxymethylated dextran matrix. |
| Sensor Chip CM5 | Biacore Sensor Chip CM5 supports a range of immobilization chemistries to capture nucleic acids, peptides, antibodies, proteins, carbohydrates, or chemical compounds for interaction analysis from research to discovery, development, and quality control. |
