Chelating Sepharose™ Fast Flow immobilized metal affinity chromatography resin
Chelating Sepharose Fast Flow is an uncharged IMAC chromatography resin for purification of proteins and peptides with affinity for metal ions. Ready to charge with your metal ion of choice for optimized selectivity.
Pack size
Overview
- Based on well-established Sepharose Fast Flow resins.
- High chemical stability enables proven CIP and sanitization protocols.
- Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Chelating resin for flexible and reliable IMAC purification
Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic, which comes immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms. The highly crosslinked agarose resin enables high flow rates and high productivity.
This immobilized metal affinity chromatography (IMAC) resin is designed to purify a wide range of proteins. It is supplied free of metal ions (uncharged), allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.
Successful use – research scale
Chelating Sepharose Fast Flow is an established tool for a range of research applications. For instance, this metal chelate affinity chromatography resin has been used to purify his-tagged proteins for the following purposes:
- Map the spatial structure of the dimeric transmembrane domain of the growth factor receptor ErbB2
- Identify distinct PIases with proven enzyme activity in E. coli
Compatible empty columns – research scale
Cytiva's solutions include a variety of empty columns for research scale. Chelating Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. Chelating Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Prepacked Chelating Sepharose Fast Flow columns – research scale
This resin is available as HiTrap columns for use with a syringe, a pump, or a chromatography system.
Prepacked Chelating Sepharose Fast Flow columns – bioprocess scale
Prepacked ReadyToProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.
