IMAC Sepharose High Performance resin
IMAC Sepharose High Performance is an uncharged IMAC resin for purifying proteins and peptides with an affinity for metal ions.
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IMAC Sepharose High Performance resin
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Overview
IMAC Sepharose High Performance is an uncharged IMAC resin for purifying proteins and peptides with an affinity for metal ions.
- Supplied uncharged, for customized metal ion charging and optimized selectivity.
- Suitable for optimizing purification of histidine-tagged (his-tagged) proteins when nickel is not the best choice of metal ion.
- High performance purification due to its 34 μm bead size.
- High binding capacity.
Uncharged resin for immobilized metal ion affinity chromatography
IMAC Sepharose High Performance is supplied free of metal ions, allowing a user to customize the charge to the target protein. Select a transition metal ion (e.g., Cu2+, Zn2+, Ni2+, or Co2+) that will bind to the covalently immobilized chelating ligand on the Sepharose.
This resin is commonly used for affinity purification of histidine-tagged proteins. However, it is also suitable for purifying native or untagged recombinant proteins.
Successful use in published research
IMAC Sepharose High Performance is trusted for protein purification in a wide body of research:
- Structural analysis of an insect β-N-Acetyl-D-hexosaminidase
- Evaluation of iron-binding properties of yeast extract hydrosylates
Compatible empty columns
Cytiva solutions include a variety of empty columns for packing. IMAC Sepharose High Performance resin is compatible for use with Tricorn, XK, and HiScale columns.
Product specifications
Showing 2 products
IMAC Sepharose High Performance, 25 mL
17092006
Discontinued. Replaced by:
IMAC Sepharose High Performance, 100 mL
17092007
Stock on request
Product availability is not guaranteed and subject to change.
