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The recombinant protein A ligand of Cyitva's MabSelect SuRe™ has been engineered to withstand 0.1–0.5 M NaOH, enabling efficient cleaning of the chromatography resin.
This resin for monoclonal antibody (mAb) purification contains a rigid, high-flow agarose matrix that delivers excellent pressure/flow properties. Like many of Cytiva's protein A chromatography resins, MabSelect SuRe™ uses oriented ligand coupling for excellent binding capacity. It exhibits low nonspecific protein binding, thereby decreasing impurity levels in the eluate pool.
MabSelect SuRe™ resin features an engineered, alkali-tolerant rProtein A ligand for mAb purification. The ligand provides greater stability than conventional protein A affinity resins in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols. The resin can withstand rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH. The enhanced alkali stability of this resin improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide, eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl.
MabSelect SuRe™ resin offers generic elution conditions for different monoclonal antibodies, which is an advantage when designing purification platform processes. This feature is also correlated to the construction of the rProtein A ligand, which has a reduced interaction with the VH3 (Fab) domain present in human antibodies.
For process-scale applications, Cytiva recommends AxiChrom™ chromatography columns for the MabSelect™ family of products.
This chromatography resin is also available in the following formats:
Prepacked ReadyToProcess™ columns are available, eliminating the need for column packing and cleaning validation.
Learn about prepacked HiScreen MabSelect SuRe, HiTrap MabSelect SuRe, ReadyToProcess MabSelect SuRe columns, PreDictor MabSelect SuRe or PreDictor Robocolumns MabSelect SuRe
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