Pharmalyte™ carrier ampholytes
Carrier ampholytes for isoelectric focusing across a pH gradient to separate charged molecules (proteins or peptides) by their isoelectric point (pI).
pH Range
Overview
Pharmalyte™ carrier ampholytes form a stable linear pH gradient with even conductivity across the gel or in the capillary. Made by co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin. For isoelectric focusing (IEF) and IEF-PAGE quality control.
- High buffer capacity per pH unit
- Linear and reproducible pH gradient
- High resolution
- Available in five broad-range and four narrow-range pH intervals
Pharmalyte™ carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in one broad-range and four narrow-range pH intervals. Each interval contains numerous ampholytes with a high buffering capacity per pH unit. Pharmalyte™ carrier ampholytes form an extremely stable linear pH gradient and exhibit even conductivity across the gel.
Learn how to optimize Pharmalyte™ batch-to-batch for pI reproducibility via our online application note.
