PreDictor Butyl Sepharose™ 4 Fast Flow prepacked 96-well plates
A prepacked 96-well plate that supports high-throughput process development (HTPD). Packed with an aliphatic hydrophobic interaction chromatography resin.
Chromatography resin volume/well
Overview
- Supports high-throughput process development (HTPD) by allowing parallel screening of chromatographic conditions using a 96-well plate format.
- Allows fast and efficient evaluation of parameters for binding/wash/elution conditions, and resins benchmarking.
- Results show good correlation with data obtained by column chromatography.
- Each well is prefilled with defined amounts of Cytiva BioProcess chromatography resins.
- Can be used with centrifugation or vacuum, manually or in automated robotic systems.
- Assist software supports the PreDictor workflow from set-up of experimental design to data evaluation.
About Butyl Sepharose 4 Fast Flow resin
Butyl Sepharose 4 Fast Flow is a well-established, standard aliphatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification of larger proteins.
Butyl Sepharose 4 Fast Flow is based on cross-linked 4% agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
About PreDictor Butyl Sepharose 4 Fast Flow plates
PreDictor plates are disposable, 96-well filter plates for parallel screening of resins and chromatographic conditions such as binding, wash, and elution conditions.
- Screen different chromatography resins simultaneously using dedicated screening plates, or more thoroughly using plates containing a single resin.
- Different applications require different amounts of resins in the wells, and we offer a wide range of PreDictor plates containing different chromatography resins at different volumes.
- Data generated using PreDictor plates show good correlation with data from chromatography columns, making the plates an excellent tool for initial screening of process conditions.
Assist software supports the PreDictor workflow from set-up of experimental design to data evaluation.
Prepacked columns and other formats
Butyl Sepharose 4 Fast Flow is available in the following formats:
Bulk resin
Loose Butyl Sepharose 4 Fast Flow resin in bottles for batch purification or packing your own columns
Process development scale – prepacked
- PreDictor RoboColumn™ Butyl Sepharose 4FF units – miniaturized columns for high-throughput process development.
- HiScreen Butyl FF columns – optimized for method and process development
Lab/research scale – prepacked for manual purification.
HiTrap Butyl FF columns – for easy use with a syringe, peristaltic pump, or chromatography system.
Lab/research scale – prepacked for automated purification and analysis
- HiTrap Butyl FF columns – for easy use with a syringe, peristaltic pump, or chromatography system.
- HiPrep Butyl FF columns – for convenient scale-up.
