Sephadex™ G-50 size exclusion resins
Well-established resin for desalting and buffer exchange of biomolecules >30 000 molecular weight
1. Particle size grade
2. Volume
Overview
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step
- Suitable for purification of DNA from small molecules by gel filtration
- BioProcess resin supported for industrial applications and well-established for desalting and buffer exchange in industrial applications
- Offered in 3 different particle sizes (Medium, Fine & Superfine)
About Sephadex™
Sephadex™ resins are prepared by cross-linking dextran with epichlorohydrin. The resins are supplied dry, except when prepacked in columns and plate format. The resins swell in aqueous solutions (see data file). Sephadex™ G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. The different types of Sephadex™ resins differ in their degree of cross-linking and hence in their degree of swelling and molecular fractionation range.
The effect of particle size
The three grades (Medium, Fine and Superfine) form the basis of a range of group separation products. Medium grade is preferred for group separations at process scale where high flow rates and low operating pressures are required. The Fine and Superfine grades are for routine lab work including preparative separations. The smaller particle sizes of the Fine and Superfine grades give shorter diffusion distances and allow for highly efficient separations at high flow rates.
| Sephadex™ G-25 | Dry particle diameter (μm) | Diameter ratio (hydrated:dry) |
|---|---|---|
| Superfine | 20 to 50 | ~ 1.7 |
| Fine | 20 to 80 | ~ 1.7 |
| Medium | 50 to 150 | ~ 1.7 |
Sephadex™ G-50 is available in different bulk pack sizes and convenient pre-packed plate and column formats.
