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The key chromatographic purification technique in the pharmaceutical industry uses highly porous micro-scale beads. These beads feature a functionalized surface that enables the separation of target molecules from complex process streams. Resin beads in packed-bed chromatography may face challenges such as diffusional and flow bottlenecks. However, a fiber-based alternative to porous beads helps to overcome these limitations and enhances productivity in chromatography.
The fiber-based Fibro technology comprises an open porous adsorbent material that features cellulose fibers for rapid mass transfer. Fibro chromatography helps resolve capacity issues of chromatography membranes. This technology has the potential to enhance process flexibility and robustness in the rapidly growing biopharmaceutical landscape. Since this chromatographic method uses the same ligands and chromatography systems as resin chromatography, it allows a swift transition into biomanufacturing facilities.Key advantages of fiber chromatography
Fibro chromatography can operate at shorter residence times compared with traditional chromatography resins. This reduces cycle times significantly from hours to minutes. The high flow rates and binding capacities of Fibro technology decrease process times for increased productivity. Reduced process development times support high throughput for biopharma applications. Fibro technology also offers improved flexibility and quick changeover time in multiproduct facilities.
Fiber chromatography FAQs
Below are answers to some frequently asked questions about fiber chromatography.How is Fibro chromatography different from conventional chromatography columns?
Fiber chromatography is a separation technique that uses high flow rates and cellulose fiber with high capacities. Fibro chromatography offers enhanced protein capture compared to resin-based chromatography. Conventional chromatography uses diffusive flow, where slow diffusion of molecules through the pores of a bead restricts mass transfer. The rigidity of porous beads also limits process flow. Fibro chromatography relies on convective flow. This allows speedy mass transfer, with pore size of matrix material being the only restricting factor. Residence times in Fibro chromatography are seconds rather than minutes.What are the applications for Fibro chromatography?
Fibro chromatography offers convenient purification solutions for research and process development. Specific applications of Fibro technology include:
- Automated high-throughput purification
- Fast purification in process development
- Scalability in downstream processing
- mAb scale-up
Monoclonal antibody (mAb purification) involves the separation of antibodies from cell culture supernatant or ascites fluid of a hybridoma cell line. The two key factors involved in monoclonal antibodies are endotoxin levels and monomer purity. Fibro PrismA technology is a protein A-based alternative to chromatography resins for the capture step in mAb purification.How much faster is Fibro chromatography?
Fibro technology enables rapid cycling chromatography, which speeds up research and process development compared with resin-based chromatography. Fiber chromatography units combine high flow rates and high binding capacities in a scalable product to allow the running of hundreds of cycles in one day. This translates to a 20-fold increase in productivity for single-use manufacturing processes.