The special design of the column, together with the well-known resin Sephadex G-25 Superfine, provides fast and reproducible separations in a convenient format.

The exclusion limit is approximately M_r 5000. This ensures group separation of proteins/peptides larger than M_r 5000 from smaller molecules.

Separations are easily performed with a syringe, a pump, or a chromatography system such as an ÄKTA system.

Successful use in published research

HiTrap Desalting columns are used in a variety of research applications, including:

• Separating rate-zonal centrifugation fractions from EGCG for the study of the salivary mucin network
• Purification of recombinant PeBL1 for the study of defense responses in Nicotiana benthamiana
• Purification with an ÄKTA system during research on the formation of otoliths in Oryzias latipes

Other Cytiva products for buffer exchange or desalting

• PD SpinTrap G-25 spin column for small sample quantities using a benchtop centrifuge – up to 180 µL sample volume
• PD MiniTrap G-25 gravity column for sample volumes up to 0.5 mL
• PD MidiTrap G-25 gravity column for sample volumes up to 1 mL
• PD-10 Desalting gravity column for sample volumes up to 2.5 mL
• HiPrep Desalting column for sample volumes up to 15 mL. For use with a peristaltic pump or a chromatography systemDifferent types of Sephadex resins are also available as bulk resins