PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.
- Flexible protocols with the ability to use gravity flow or centrifugation.
- Convenient desalting and buffer exchange of sample volumes of 0.1 mL to 0.5 mL for gravity and 0.2 mL to 0.5 mL with centrifugation.
- Replaces dialysis for desalting protein, which saves time and reduces labor.
- Filled with Sephadex G-25 Superfine resin.
Column for desalting and buffer exchange of proteins
PD MiniTrap G-25 is designed for convenient desalting of biological samples. For increased flexibility, the protein desalting column can be used with either gravity or centrifugation.Read more
When using the gravity protocol, clean-up of the sample is simple. One or several columns can be connected in parallel, and a purification system is not needed. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge.The eluted sample will have minimal dilution.
Four adapters are included in each product pack. The adapters simplify centrifugation of these protein desalting columns.
The exclusion limit is approximately Mr 5000. This ensures group separation of proteins/peptides larger than Mr 5000 from smaller molecules.
Successful use in published research
PD MiniTrap G-25 are used in a variety of research applications, including:
- Buffer exchange during in vitro assembly of catalase
- Removal of DTT during study of thiosulfate transfer in Metallosphaera cuprina
- Imidazole removal during study of disparate ligand binding sites in the human P2Y1 receptor
Other Cytiva products for buffer exchange or desalting
- PD SpinTrap G-25 spin column for small sample quantities using a benchtop centrifuge – up to 180 µL sample volume
- PD MidiTrap G-25 gravity column for sample volumes up to 1 mL
- PD-10 Desalting gravity column for sample volumes up to 2.5 mL
- HiTrap Desalting columns for sample volumes up to 1.5 mL. For use with a syringe, a peristaltic pump, or a chromatography system
- HiPrep Desalting column for sample volumes up to 15 mL. For use with a laboratory pump or a chromatography system
- PD MultiTrap G-25 for high-throughput desalting and buffer exchange of multiple samples
- This resin is available as bulk resin
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